Pharmacokinetic and biodistribution study following systemic administration of Fospeg® - a Pegylated liposomal mTHPC formulation in a murine model
| Autor: | Susanna Gräfe, Katarina Svanberg, Haiyan Xie, Stefan Andersson-Engels, Maria Kyriazi, Pontus Svenmarker, Niels Bendsoe, Johan Axelsson |
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| Rok vydání: | 2013 |
| Předmět: |
Biodistribution
Fluorescence-lifetime imaging microscopy Liposome General Engineering General Physics and Astronomy General Chemistry Pharmacology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Pharmacokinetics chemistry Chlorin Systemic administration General Materials Science Photosensitizer Ex vivo |
| Zdroj: | Journal of Biophotonics. 8:142-152 |
| ISSN: | 1864-063X |
| DOI: | 10.1002/jbio.201300133 |
| Popis: | in Undetermined Fospeg® is a newly developed photosensitizer formulation based on meso-tetra(hydroxyphenyl)chlorin (mTHPC), with hydrophilic liposomes to carry the hydrophobic photosensitizer to the target tissue. In this study the pharmacokinetics and biodistribution of Fospeg® were investigated by high performance liquid chromatography at various times (0.5-18 hours) following systemic i.v. administration. As a model an experimental HT29 colon tumor in NMRI nu/nu mice was employed. Our study indicates a higher plasma peak concentration, a longer circulation time and a better tumor-to-skin ratio than those of Foslip®, another liposomal mTHPC formulation. Data from ex vivo tissue fluorescence and reflectance imaging exhibit good correlation with chemical extraction. Our results have shown that optical imaging provides the potential for fluorophore quantification in biological tissues. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). (Less) |
| Databáze: | OpenAIRE |
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