Free radical scavenging ability and structure of a 90-kDa protein from the scallop shell

Autor:	Kohji Ono, Manabu Fukuda, Yasushi Hasegawa, Takuya Mitsuhashi
Rok vydání:	2013
Předmět:	chemistry.chemical_classification chemistry.chemical_compound chemistry Biochemistry Superoxide DPPH Scallop Aquatic Science Free radical scavenger Glycoprotein Mass spectrometry Scavenging Amino acid
Zdroj:	Fisheries Science. 79:495-502
ISSN:	1444-2906 0919-9268
DOI:	10.1007/s12562-013-0616-7
Popis:	We have previously reported that the scallop shell extract possesses free radical scavenging activity. In this study, we isolated a glycoprotein with a molecular weight of approximately 90 kDa (named 90-kDa protein) which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion radical scavenging, reducing, and ferrous ion-chelating activities. Amino acid composition analysis showed that the 90-kDa protein was rich in Asx (Asp or Asn), Ser and Gly residues. A BLAST search of partial amino acid sequences determined by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry revealed that the 90-kDa protein is a novel protein. The 90-kDa protein is a yellow protein that contains fluorescent substances. To the best of our knowledge, this is the first report of a radical scavenging protein from the scallop shell.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::41ac14bfd605199b3e8f3e5cabba87ca https://doi.org/10.1007/s12562-013-0616-7 Zobrazit plný text záznamu Plný text ve formátu PDF