Electrochemical Behavior of Cytochrome c552 from a Psychrophilic Microorganism

Autor:	Marisa C. Buzzeo, John S. Magyar, Olga M. Sokolovskaya
Rok vydání:	2014
Předmět:	Cytochrome biology Chemistry Context (language use) Electrochemistry Redox Surfaces Coatings and Films Electronic Optical and Magnetic Materials Electron transfer General Energy Bioremediation Biochemistry Electrode biology.protein Biophysics Physical and Theoretical Chemistry Psychrophile
Zdroj:	The Journal of Physical Chemistry C. 118:18829-18835
ISSN:	1932-7455 1932-7447
DOI:	10.1021/jp501146e
Popis:	Psychrophilic organisms play a significant role in global nutrient cycling and bioremediation, and thus there is great interest in understanding the details of their cellular processes. Here, we report the first electrochemical measurements of cytochrome c552 isolated from the model psychrophile, Colwellia psychrerythraea. Quasi-reversible redox behavior is observed at thiol-modified and bare gold electrodes with midpoint potentials of 247 and 277 mV vs SHE, respectively. Comparison of the voltammetric response on the two substrates illustrates the need for a modified surface to direct protein orientation and facilitate electron transfer in a reproducible manner. Temperature-dependence measurements of the current profile illustrate the protein’s ability to participate in electron transfer reactions over a 50° range, which is supported by recent structural evidence of enhanced flexibility. These electrochemical findings allow the behavior of this psychrophilic cytochrome to be evaluated in the context of m...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4199f75d1612fb50dbb04f38a1fc17af https://doi.org/10.1021/jp501146e Zobrazit plný text záznamu