Enzymatic esterification of (−)-menthol with lauric acid in isooctane by sorbitan monostearate-coated lipase from Candida rugosa

Autor: Guldem Ustun, Melek Tüter, Banu Babali
Rok vydání: 2001
Předmět:
Zdroj: Journal of the American Oil Chemists' Society. 78:173-175
ISSN: 1558-9331
0003-021X
Popis: Esterification of (−)-menthol and (±)-menthol with lauric acid in isooctane was successfully catalyzed by a commercial nonioic surfactant (sorbitan monosterate)-coated lipase from Candida rugosa (Lipase AY “Amano” 30) at the molar ratio of 1∶1 and at 35°C using 1.5 g enzyme/g (−)-menthol and 0.1-g molecular sieves. After 1 h, molar conversion of (−)-menthol reached 81%. Equilibrium was reached after ca. 4 h, giving a (−)-menthol molar conversion of 94%. Under the same conditions, native lipase catalyzed the esterification of (−)-menthol and lauric acid to yield a molar conversion of 93% after 72 h. Coating the lipase with sorbitan monosterate increased the esterification rates of both (−)-menthol and (±)-menthol with lauric acid. After 6 h, the molar conversions of (−)-menthol and (±)-menthol were 94, and 62%, respectively.
Databáze: OpenAIRE
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