A novel, bedside technique to rapidly identify umbilical cord blood units with high total nucleated cell numbers
| Autor: | Werner Krenger, Daniel Surbek, Gabriela M. Baerlocher, Elke Beutler, Shamsiya Stettler, Anna Margareta Wagner, Judith Herbst |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
business.industry Immunology High cell Hematology Cost efficacy 030204 cardiovascular system & hematology musculoskeletal system Umbilical cord Surgery Andrology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Nucleated cell Cord blood Placenta medicine Immunology and Allergy Blood units business Blood processing 030215 immunology |
| Zdroj: | Transfusion. 56:1406-1412 |
| ISSN: | 0041-1132 |
| DOI: | 10.1111/trf.13610 |
| Popis: | BACKGROUND With increasing demand for umbilical cord blood units (CBUs) with total nucleated cell (TNC) counts of more than 150 × 10(7) , preshipping assessment is mandatory. Umbilical cord blood processing requires aseptic techniques and laboratories with specific air quality and cleanliness. Our aim was to establish a fast and efficient method for determining TNC counts at the obstetric ward without exposing the CBU to the environment. STUDY DESIGN AND METHODS Data from a total of 151 cord blood donations at a single procurement site were included in this prospective study. We measured TNC counts in cord blood aliquots taken from the umbilical cord (TNCCord ), from placenta (TNCPlac ), and from a tubing segment of the sterile collection system (TNCTS ). TNC counts were compared to reference TNC counts in the CBU which were ascertained at the cord blood bank (TNCCBU ). RESULTS TNCTS counts (173 ± 33 × 10(7) cells; calculated for 1 unit) correlated fully with the TNCCBU reference counts (166 ± 33 × 10(7) cells, Pearson's r = 0.97, p |
| Databáze: | OpenAIRE |
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