Stored red blood cell susceptibility to in vitro transfusion-associated stress conditions is higher after longer storage and increased by storage in saline-adenine-glucose-mannitol compared to AS-1
| Autor: | Amrita Sran, Rosemary L. Sparrow, Esther Bandala-Sanchez, Kasey Sze-Kei Chan, Diana Mittag, Olivier Huet, William Xu, Martin Boland |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.diagnostic_test
Chemistry Immunology hemic and immune systems Hematology Phosphatidylserine Adhesion medicine.disease_cause Flow cytometry Andrology chemistry.chemical_compound Red blood cell medicine.anatomical_structure Biochemistry medicine Immunology and Allergy Mannitol Cell adhesion Oxidative stress circulatory and respiratory physiology Whole blood medicine.drug |
| Zdroj: | Transfusion. 55:2197-2206 |
| ISSN: | 0041-1132 |
| Popis: | BACKGROUND Biochemical changes induced in red blood cells (RBCs) during storage may impair their function upon transfusion. Transfusion-associated stresses may further amplify storage lesion effects including increased phosphatidylserine (PS) exposure at the RBC membrane, microparticle (MP) release, and adhesion to endothelial cells (ECs). RBC stress susceptibility in vitro was investigated in relation to storage time and additive solution. STUDY DESIGN AND METHODS Leukoreduced whole blood donations (n = 18) were paired, mixed, and resplit before separating the RBCs for storage in saline-adenine-glucose-mannitol (SAGM) or AS-1. Samples were taken after 3, 21, or 35 days. For oxidative stress treatment, RBCs were exposed to 0.5 mmol/L tert-butylhydroperoxide. Transfusion-associated stress was simulated by overnight culture at 37 °C with plasma containing inflammatory mediators. PS exposure and MPs were measured by flow cytometry and adhesion to ECs was tested under flow conditions. PS specificity of adhesion was tested by blocking with PS-containing lipid vesicles. RESULTS Oxidative stress induced significantly higher PS exposure and adhesion to ECs in RBCs stored for 35 days compared to 3 days (p |
| Databáze: | OpenAIRE |
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