| Autor: |
Rukiye Akyol, Fikriye Milletli Sezgin, Mustafa Kara, Lokman Hızmalı, Veysel Akça, Zeynep Köylü, Fatma Çelik, Yahya Şahin, Selda Songur Dağli, Işıl Sayhan, Sercan Eroğlu, Yavuz Simsek, Barış Çıplak |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.21203/rs.3.rs-395951/v1 |
| Popis: |
Purpose: ACE 2 RNA expression has been detected in organs of the female reproductive tract, suggesting that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could potentially infect female reproductive organs. In this study, we investigated the presence of SARS-CoV-2 virus in the cervicovaginal fluid.Materials and Methods: Our study included 31 female patients aged 18–65 years. The presence of SARS-COV-2 RNA was investigated by RT-PCR in two separate cervicovaginal swab samples collected from patients 14 days apart. Viral RNA was extracted using viral nucleic acid buffer (vNAT) solution, and SARS-COV-2 RNA was analyzed using Bio-speedy SARS-CoV-2 RT-qPCR kits in Bio-Rad CFX96 TouchTM device.Results: The first and second cervical swab samples were collected from 22 of 31 patients 14 days apart. The first cervical swab sample was collected from 9 patients; however, the second swab sample could not be collected after 14 days. SARS-COV-2 RNA result was negative in 100% of a total of 53 cervicovaginal swab samples collected. Moreover, the SARS-COV-2 RNA result was negative in the nasopharyngeal swab of babies after delivery in three pregnant women.Conclusion: Negative SARS-COV-2 RNA results in cervicovaginal swab samples suggest that there is no sexual transmission of COVID-19 and no vertical transmission during pregnancy. However, the number of studies conducted on this subject and the sample size examined are still insufficient. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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