| Popis: |
We present a reverse phase liquid chromatographic assay involving pre-column o-phthalaldehyde derivatization and fluorescence detection with the capability of measuring dezocine in human serum and urine for pharmacokinetic or forensic purposes. It features a simple liquid-liquid extraction scheme using hexane, conservative detection limits of 1 ng/ml, linearity to 1000 ng/ml, precision of 10% (CV%) in the low ng/ml region, approximate chromatography time of 25 minutes and the use of a new internal standard, WY-19083. |
