| Popis: |
(A) pCAMBIA-1300 based construct used for overexpressing maize cytosolic carbonic anhydrase (CA) gene in rice. Maize PEPC gene promoter was used to drive the expression of CA and 35S prmomter driven bar, which encodes DPAT (demethylphosphinothricin acetyltransferase), was used for selection of transgenic plants by the herbicide bialophos. (B) Southern blot analysis of bar in wild type (WT) and selected T0 CKX2-overexpressiom transgenic lines (1, 2, 3, 5, 9, 10) containing the maize carbonic anhydrase gene. Plasmid DNA (P) was used as a positive control. Genomic DNA was digested with HindIII, resolved by electrophoresis and hybridized with DIG labeled bar probe. (C) Location of insert fragment on rice chromosome 1. (DOC 108 kb) |
