Determination of Cu, Mn, Fe, and Ca in six regions of normal human brain, by atomic absorption spectroscopy

Autor:	Allen N, W J Goldberg
Rok vydání:	1981
Předmět:	Putamen Biochemistry (medical) Clinical Biochemistry Analytical chemistry chemistry.chemical_element Substantia nigra Manganese Calcium Copper Methyl isobutyl ketone chemistry.chemical_compound Globus pallidus nervous system chemistry Acetylene Nuclear chemistry
Zdroj:	Clinical Chemistry. 27:562-564
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/27.4.562
Popis:	A method is reported for measuring copper, manganese, iron, and calcium in brain tissue. We solubilized 100-mg samples of brain tissue with Soluene-350 and diluted the mixtures with methyl isobutyl ketone. Copper and manganese concentrations were measured by using a carbon rod atomizer, iron and calcium concentrations by using an air/acetylene and nitrous oxide/acetylene flame, respectively. The basal ganglia (caudate, putamen, globus pallidus, and substantia nigra) contained high concentrations of iron (10 to 19 mumol/g dry wt) as compared to concentrations found in the cortex (4 mumol/g) or the hippocampus (2.8 mumol/g). Copper exhibited a similar distribution pattern among the regions studied; 526 to 1140 mumol/g (dry wt) in the basal ganglia vs 496 mumol/g in the cortex and 426 mumol/g in the hippocampus. Manganese and calcium were uniformly distributed in the regions examined.
Databáze:	OpenAIRE
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