Polynucleotide phosphorylase and RNA helicase CshA cooperate in Bacillus subtilis mRNA decay
| Autor: | Shivani Chhabra, Elizabeth Salvo, David H. Bechhofer, Denise Laspina, Shakti Ingle, Bo Liu |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Messenger RNA RNA Cell Biology Processivity Bacillus subtilis Biology biology.organism_classification RNA Helicase A Nucleic acid secondary structure Cell biology 03 medical and health sciences 0302 clinical medicine 030220 oncology & carcinogenesis Exoribonuclease Polynucleotide phosphorylase Molecular Biology 030304 developmental biology |
| Zdroj: | RNA Biology. 18:1692-1701 |
| ISSN: | 1555-8584 1547-6286 |
| DOI: | 10.1080/15476286.2020.1864183 |
| Popis: | Polynucleotide phosphorylase (PNPase), a 3' exoribonuclease that degrades RNA in the 3'-to-5' direction, is the major mRNA decay activity in Bacillus subtilis. PNPase is known to be inhibited in vitro by strong RNA secondary structure, and rapid mRNA turnover in vivo is thought to require an RNA helicase activity working in conjunction with PNPase. The most abundant RNA helicase in B. subtilis is CshA. We found for three small, monocistronic mRNAs that, for some RNA sequences, PNPase processivity was unimpeded even without CshA, whereas others required CshA for efficient degradation. A novel colour screen for decay of mRNA in B. subtilis was created, using mRNA encoded by the slrA gene, which is degraded from its 3' end by PNPase. A significant correlation between the predicted strength of a stem-loop structure, located in the body of the message, and PNPase processivity was observed. Northern blot analysis confirmed that PNPase processivity was greatly hindered by the internal RNA structure, and even more so in the absence of CshA. Three other B. subtilis RNA helicases did not appear to be involved in mRNA decay during vegetative growth. The results confirm the hypothesis that efficient 3' exonucleolytic decay of B. subtilis RNA depends on the combined activity of PNPase and CshA. |
| Databáze: | OpenAIRE |
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