Alkoholdehydrogenase (ADH) in Hefezellen I. Grundplasmatische, mitochondriale und nucleäre ADH in Saccharomyces carlsbergensis und Kluyveromyces fragilis

Autor:	R. May, W. Künkel
Rok vydání:	2007
Předmět:	integumentary system biology Biochemistry urogenital system Chemistry Yield (chemistry) Genetics General Medicine Kluyveromyces fragilis biology.organism_classification Applied Microbiology and Biotechnology Saccharomyces hormones hormone substitutes and hormone antagonists
Zdroj:	Zeitschrift für allgemeine Mikrobiologie. 16:529-536
ISSN:	1521-4028 0044-2208
DOI:	10.1002/jobm.19760160705
Popis:	When grown in a medium containing lactat, Saccharomyces carlsbergensis produces 5 times more ground-plasmatic ADH than Kluyveromyces fragilis. Upon gelelectrophoresis, K. fragilis exhibits 7 bands while S. carlsbergensis shows only one. In a polyacrylamid gradient, the ADH's of both strains yield one band, the position of which corresponds to a molecular weight of about 160 000 D. In cell homogenates and mitochondrial fractions of S. carlsbergensis treated with ultrasound, an ADH is detected which exhibits 3 subbands and a molecular weight greater than 1 Megadalton. This ADH does not occur in K. fragilis. Mitochondrial fractions from K. fragilis contain an ADH, the electrophoretical mobility of which is identical to that of the ADH of the groundplasma. Nuclei of S. carlsbergensis also possess ADH whereas those of K. fragilis probably do not.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::41159604ffb7d0f45ad197b29ec70ff4 https://doi.org/10.1002/jobm.19760160705 Zobrazit plný text záznamu Plný text