| Autor: |
Tamara Tretskaya, Tatyana Yashina, Betty H. Robertson, Michael O. Favorov, Bhawna Khanna |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
Viral Hepatitis and Liver Disease ISBN: 9784431682578 |
| DOI: |
10.1007/978-4-431-68255-4_75 |
| Popis: |
Genotypes of hepatitis C virus (HCV) have been defined based upon many different genetic comparisons. These have ranged from comparison of the entire HCV genome to comparison of partial sequences from either the 5′ nontranslated (NTR), core, NS3, or NS5 regions. Based upon a comparison of the genetic diversity among five published strains. We selected the core region for genetic comparison of unknown strains since it represented the least variability within the open reading frame. Nested PCR amplification of the core region of 40 individual serum samples from the former Soviet Union resulted in a positive band in 38 samples after 60 cycles of amplification. The cDNA fragments from 12 samples were purified on an acrylamide gel and then sequenced. Nucleotide sequence comparison revealed that 11 of the 12 were sequences related to each other and differed from other published sequences in this region by approximately 15%. The remaining strain was 95% homologous with strains found in the United States and Europe. These results reveal a new genotype of HCV was found in the majority of samples from Moscow, Tajikistan, and Ukraine. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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