Evaluation of in vitro susceptibility of Gram-positive pathogens from a tertiary care hospital in Singapore to a novel oxazolidinone, tedizolid, by a gradient diffusion method and broth microdilution
| Autor: | Long Jen Mee, Shawn Vasoo, Saugata Choudhury, Lee Kar Mun, Subodha Samanthini Wickramasinghe, Lee Shin Jia, Esme Ng Chu Xuan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gradient Diffusion Method biology business.industry Broth microdilution General Medicine biochemical phenomena metabolism and nutrition Antimicrobial biology.organism_classification medicine.disease_cause Enterococcus faecalis Pathology and Forensic Medicine Microbiology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Antibiotic resistance chemistry Staphylococcus aureus 030220 oncology & carcinogenesis Linezolid Medicine Tedizolid business |
| Zdroj: | Journal of Clinical Pathology. 72:181-184 |
| ISSN: | 1472-4146 0021-9746 |
| Popis: | We compared the in vitro antimicrobial activities of tedizolid and linezolid on the Sensititre broth microdilution system for Gram-positive cocci isolates (n=146) from skin and skin structure infections and bloodstream infections, bronchoalveolar lavage and sputum. These pathogens included 40 methicillin-resistant Staphylococcus aureus, 38 coagulase-negative staphylococci, 20 Enterococcus faecalis and 48 beta-haemolytic Streptococcus spp. Susceptibility was simultaneously determined for 48 vanA vancomycin-resistant enterococci isolates 2013–2016 from rectal swabs (23 E. faecalis and 25 E. faecium, of which 4 were linezolid-non-susceptible). MIC90s for tedizolid were fourfold to eightfold lower than linezolid on the Sensititre and ranged from 0.12 to 0.5 µg/mL for the different pathogen groups. All isolates were susceptible to tedizolid except two vanA E. faecium strains (MICs of 1 and 2 µg/mL, respectively). Categorical and essential agreement for tedizolid were 99.48% and 92%, respectively, between Liofilchem gradient diffusion and Sensititre methods. Overall, the drug exhibited excellent activity against the surveyed Gram-positive pathogens. |
| Databáze: | OpenAIRE |
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