Extracts From Leaves of Allamanda blanchetti Inducing Mechanism of Defense to Diseases in Sugarcane

Autor: Bruno Oliveira de Veras, Clébia Maria Alves de Almeida, Fernanda Granja da Silva Oliveira, Louise Melo de Souza Oliveira, Márcia Vanusa da Silva, Rildo S. B. Coelho, Maria Tereza dos Santos Correia, Juciara Carneiro Gouveia Tenório, Alexandre Gomes da Silva, Leonardo Sousa Cavalcanti
Rok vydání: 2019
Předmět:
Zdroj: Journal of Agricultural Science. 11:282
ISSN: 1916-9760
1916-9752
DOI: 10.5539/jas.v11n3p282
Popis: This research aims to analyze the effect of the extract from a native plant from Caatinga, Allamanda blanchetti, in the induction of resistance mechanisms in a sugarcane variety smut-susceptible. Initially, it carried out a phytochemical analysis to know the main plant compounds used in this study. Diverse chemicals content in ethanolic extract from A. blanchetii were detected by thin-layer chromatography (TLC). Flavonoids, were more abundant compounds following by terpenes, stereroids and saponins. Under greenhouse conditions the sugarcane plants, SP-791011 (smut-susceptible), were sprayed with extracts from A. blanchetti extracted at cold at concentrations of 1000 ppm and acilbenzolar-S-metil (ASM) (100 mg/L). Leaves were collected at 0, 24 and 48 hours after spraying and used in the RT-PCR analysis for to identify the defense gene expression. Change in gene expression were observed in the different treatments, especially in the expression of pathogenesis-related (PR) genes. The extract of A. blanchetti induced an increase in the glucanase expression and was more effective than ASM inducer. SNPR1 gene show increased in the two treatment. The results indicate that A. blanchetti extracts was able to activate the resistance mechanism as observed in resistant plants. This paper is the first report about the use of Caatinga natural plant extracts inducing resistance genes against Sporisorium scitamineum in sugarcane susceptible genotype.
Databáze: OpenAIRE
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