Effect of Skin Irritation Induced by Potassium Hydroxide and Trichloroacetic Acid on in vitro Percutaneous Penetration of Estradiol

Autor:	Leanne L. Zhang, Howard I. Maibach, Marylou C. Thelmo, Hanafi Tanojo
Rok vydání:	2002
Předmět:	Potassium hydroxide Chromatography Public Health Environmental and Occupational Health Dermatology Percutaneous penetration In vitro chemistry.chemical_compound Skin irritation chemistry In vivo parasitic diseases Organic chemistry Irritant dermatitis Trichloroacetic acid
Zdroj:	Exogenous Dermatology. 1:102-106
ISSN:	1424-4624 1424-4616
DOI:	10.1159/000058341
Popis:	Background: In vivo manifestations of irritant dermatitis to two known irritants, potassium hydroxide (KOH) and trichloroacetic acid (TCA), are studied in vitro to define the mechanism by which these processes might alter flux. Objective: To study the change in the in vitro percutaneous penetration of estradiol on excised skin pretreated with KOH or TCA. Methods: Pretreatment of fresh human skin with various concentrations of KOH for 2 h or of TCA for 24 h. Penetration of [14C]estradiol through the pretreated skin was monitored using in vitro diffusion cells. Untreated skin was used as control. Results: There was a significant decrease in skin permeability of estradiol on KOH pretreated skins compared to control. Comparisons between 0.05, 0.1 and 0.2% KOH were significant (p < 0.05). Skin permeability of estradiol decreased significantly after 7.5% TCA pretreatment compared to control, 20 and 30% TCA (p < 0.05). Conclusion: KOH and TCA damage the skin barrier property, thus blocking the permeation of lipophilic compounds. The damage is dose dependent. However, there may be more than one mechanism involved.
Databáze:	OpenAIRE
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