Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Autor:	Huanhuan Yang, Xianghua Tang, Bo Xu, Zunxi Huang, Xue Li, Yunjuan Yang, Tianbao Luo, Junjun Li
Rok vydání:	2018
Předmět:	biology Moisture Bran Chemistry Dry basis Rhizopus oryzae General Medicine biology.organism_classification Applied Microbiology and Biotechnology Solid-state fermentation Rhizopus Fermentation Food science Mycelium Biotechnology
Zdroj:	Journal of Microbiology and Biotechnology. 28:1865-1875
ISSN:	1738-8872 1017-7825
DOI:	10.4014/jmb.1805.05023
Popis:	Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, μ of mycelium reached to 0.15 h-1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.
Databáze:	OpenAIRE
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