Simultaneous Determination of Hyoscine Butylbromide and Ketoprofen in Pharmaceutical Preparations by Spectrophotometric and Liquid Chromatographic Methods
| Autor: | Yasser S. El-Saharty, Mohamed I Refaat, S. Z. El-khateeb, Fadia H Metwally |
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| Rok vydání: | 2007 |
| Předmět: |
Pharmacology
Ketoprofen Accuracy and precision Chromatography medicine.diagnostic_test Resolution (mass spectrometry) Hyoscine butylbromide Chemistry Analytical chemistry Analytical Chemistry Spectrophotometry Standard addition medicine Environmental Chemistry Agronomy and Crop Science Food Science medicine.drug |
| Zdroj: | Journal of AOAC INTERNATIONAL. 90:102-112 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/90.1.102 |
| Popis: | A binary mixture of hyoscine butylbromide and ketoprofen was determined by 4 different methods. The first involved determination of hyoscine butylbromide and ketoprofen using the ratio-spectra first-derivative spectrophotometric technique at 211 and 234 nm over the concentration ranges of 2-14 and 5-45 μg/mL with mean accuracies 99.84 ± 0.92 and 99.98 ± 0.64%, respectively. The second method utilized second-derivative spectrophotometry over the concentration ranges of 2-14 and 5-35 μg/mL with mean accuracies 99.32 ± 1.06 and 99.55 ± 1.15%, respectively. The third method was based on the resolution of the 2 components by bivariate calibration depending on a simple and rapid mathematical algorithm and quantitative evaluation of the absorbances at 206 and 254 nm over concentration ranges of 2-16 and 5-35 μg/mL; mean accuracies of 100.21 ± 1.30 and 100.19 ± 1.07% were obtained for hyoscine butylbromide and ketoprofen, respectively. The fourth method was reversed-phase liquid chromatography using 0.05 M ammonium dihydrogen phosphateacetonitrilemethanol (20 + 30 + 6, v/v) as the mobile phase with ultraviolet detection at 220 nm over concentration ranges of 1-90 and 5-70 μg/mL; mean accuracies were 99.92 ± 1.02 and 99.61 ± 0.98%, respectively. The suggested procedures were checked using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical preparations. The methods retained their accuracy and precision when the standard addition technique was applied. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by the manufacturer's method. |
| Databáze: | OpenAIRE |
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