Rhizobium meliloti Tn5 induced auxotrophic mutants responding to different biosynthetic intermediates
| Autor: | Hassan Ali, Max Béchet, Christian Niel, Jean-Bernard Guillaume |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Transposable element
chemistry.chemical_classification Genetics Methionine biology Auxotrophy Immunology Genetic transfer Mutant General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Applied Microbiology and Biotechnology Microbiology Amino acid chemistry.chemical_compound Plasmid chemistry bacteria Rhizobium Molecular Biology |
| Zdroj: | Canadian Journal of Microbiology. 30:507-511 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m84-075 |
| Popis: | Using a suicide plasmid as a vector, the kanamycin–neomycin resistance conferring transposon Tn5 was introduced into the genome of the two Rhizobium meliloti strains 444 and M28str. Among clones carrying random Tn5 insertions, about 0.3% auxotrophic mutants were isolated from each strain. Tn5 was found to integrate preferentially into genes involved in syntheses of adenine, arginine, leucine, pyrimidines, sulphur-containing amino acids, and tryptophan. Growth responses to biosynthetic intermediates uncovered four and two groups of methionine- and tryptophan-requiring mutants, respectively. |
| Databáze: | OpenAIRE |
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