Comparison of inoculation methods with Mycosphaerella brassicicola on Brassica oleracea var. capitata: ascospores versus mycelial fragments
Autor: | H. D. Frinking, J.E. van den Ende |
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Rok vydání: | 1993 |
Předmět: | |
Zdroj: | Netherlands Journal of Plant Pathology. 99:69-81 |
ISSN: | 1573-8469 0028-2944 |
DOI: | 10.1007/bf03041397 |
Popis: | Ascospores can be collected from dried leaves of white cabbage from the previous season, carrying lesions of the fungus. Discharge of ascospores is stimulated by light and takes place within a broad temperature range (5–20 °C) under humid conditions. A method is described to isolate single ascospores, or to collect sufficient ascospores for small inoculation experiments. In order to screen large numbers of plants under controlled conditions, mycelial fragments can be used as inoculum. Using mycelial fragments requires a long (4–5 days) duration of leaf wetness necessary for infection. Ascospores infected the host plant with a much shorter duration of leaf wetness ( |
Databáze: | OpenAIRE |
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