The ABCs of viral hepatitis that define biomarker signatures of acute viral hepatitis
Autor: | Amal A. Abbas, Charlotte Soneson, Armanda Casrouge, Mostafa K. Mohamed, Magnus Fontes, Mohamed Abdel-Hamid, Mai El-Daly, Noha Sharaf Eldin, Darragh Duffy, Melissa E. Laird, Arnaud Fontanet, Jérémie Decalf, Matthew L. Albert, Rasha Mamdouh, Mona Rafik, Lénaig Le Fouler |
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Rok vydání: | 2014 |
Předmět: |
medicine.medical_specialty
viruses Virus 03 medical and health sciences Liver disease 0302 clinical medicine Immune system Internal medicine medicine Functional studies 030304 developmental biology 0303 health sciences Hepatology business.industry virus diseases Hepatitis A medicine.disease Blood proteins Virology digestive system diseases 3. Good health Immunology 030211 gastroenterology & hepatology business Viral hepatitis |
Zdroj: | Hepatology. 59:1273-1282 |
ISSN: | 0270-9139 |
Popis: | Viral hepatitis is the leading cause of liver disease worldwide and can be caused by several agents, including hepatitis A (HAV), B (HBV), and C (HCV) virus. We employed multiplexed protein immune assays to identify biomarker signatures of viral hepatitis in order to define unique and common responses for three different acute viral infections of the liver. We performed multianalyte profiling, measuring the concentrations of 182 serum proteins obtained from acute HAV- (18), HBV- (18), and HCV-infected (28) individuals, recruited as part of a hospital-based surveillance program in Cairo, Egypt. Virus-specific biomarker signatures were identified and validation was performed using a unique patient population. A core signature of 46 plasma proteins was commonly modulated in all three infections, as compared to healthy controls. Principle component analysis (PCA) revealed a host response based upon 34 proteins, which could distinguish HCV patients from HAV- and HBV-infected individuals or healthy controls. When HAV and HBV groups were compared directly, 34 differentially expressed serum proteins allowed the separation of these two patient groups. A validation study was performed on an additional 111 patients, confirming the relevance of our initial findings, and defining the 17 analytes that reproducibly segregated the patient populations. Conclusions: This combined discovery and biomarker validation approach revealed a previously unrecognized virus-specific induction of host proteins. The identification of hepatitis virus specific signatures provides a foundation for functional studies and the identification of potential correlates of viral clearance. (Hepatology 2014;59:1273-1282) |
Databáze: | OpenAIRE |
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