Evaluation of natural dyes Curcuma longa and Nyctanthes arbor ‐ tristis with different mordants on plant tissues under fluorescence microscopy
| Autor: | Krishnan Sellappan, Nikita G. Shet Verenkar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Histology
Nyctanthes biology Chemistry food and beverages Mordant 030206 dentistry 02 engineering and technology 021001 nanoscience & nanotechnology biology.organism_classification Fluorescence Staining Rhizome 03 medical and health sciences Medical Laboratory Technology 0302 clinical medicine Fluorescence microscope Anatomy Curcuma 0210 nano-technology Instrumentation Natural dye Nuclear chemistry |
| Zdroj: | Microscopy Research and Technique. 84:902-911 |
| ISSN: | 1097-0029 1059-910X |
| Popis: | The study reveals the effect of natural dyes Curcuma longa (rhizome) and Nyctanthes arbor-tristis (corolla tube) on various plant tissues under fluorescence microscopy. The results indicated that the auto-fluorescence was seen in some of the tissues of unstained monocot and dicot stem sections. The use of Curcuma longa dye along with different chemical mordants leads to various colors and intensity of fluorescence under different excitation filters (violet: 400-450; blue: 450-500; green: 500-570; yellow: 570-610 and red: 610-750 nm) examined. The extract of Nyctanthes arbor-tristis along with the mordants lead to bluish-orange fluorescence of vascular tissues under violet excitation filter. Among the dyes evaluated, Nyctanthes arbor-tristis showed better fluorescence than the Curcuma longa dye. Hence, the dye extracted from the corolla tube of Nyctanthes arbor-tristis is a potential natural dye and could be used as biological stain for staining plant tissues. |
| Databáze: | OpenAIRE |
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