Influence of Buffer Composition and Calcium Chloride on GdnHCl Denaturation of Bacillus licheniformis α-Amylase
| Autor: | Saad Tayyab, Adawiyah Suriza Shuib, Cheau Y. Tan, Salanee Kandandapani |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine HEPES Circular dichroism Chromatography biology Sodium chemistry.chemical_element General Medicine biology.organism_classification 01 natural sciences Biochemistry MOPS 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Structural Biology 010608 biotechnology biology.protein Denaturation (biochemistry) Amylase Bacillus licheniformis Guanidine |
| Zdroj: | Protein & Peptide Letters. 23:537-543 |
| ISSN: | 0929-8665 |
| DOI: | 10.2174/0929866523666160303112554 |
| Popis: | The influence of buffer composition on the conformational stability of native and calciumdepleted Bacillus licheniformis α-amylase (BLA) was investigated against guanidine hydrochloride (GdnHCl) denaturation using circular dichroism, fluorescence and UV-difference spectroscopy. Differential effect of buffer composition on GdnHCl denaturation of BLA was evident from the magnitude of these spectral signals, which followed the order: sodium phosphate > Tris-HCl > HEPES > MOPS. These effects became more pronounced with calcium-depleted BLA. Sephacryl S-200 gel chromatographic results showed significant BLA aggregation in the presence of 6 M GdnHCl. |
| Databáze: | OpenAIRE |
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