| Autor: |
Moises Freitas-Andrade, Cesar H. Comin, Peter Van Dyken, Julie Ouellette, Joanna Raman-Nair, Nicole Blakeley, Qing Yan Liu, Sonia Leclerc, Youlian Pan, Ziying Liu, Micaël Carrier, Karan Thakur, Alexandre Savard, Gareth M. Rurak, Marie-Ève Tremblay, Natalina Salmaso, Luciano Da F. Costa, Gianfilippo Coppola, Baptiste Lacoste |
| Rok vydání: |
2023 |
| DOI: |
10.1101/2023.04.29.538807 |
| Popis: |
Astrocytes are intimately linked with brain vessels, a relationship that is critical for neuronal health and function. However, astroglial factors driving these physical and functional associations during postnatal brain development have yet to be identified. We characterized structural and transcriptional changes in mouse cortical astrocytes and microvessels during the first two postnatal weeks and found that high-mobility group box 1 (Hmgb1), normally upregulated with injury and involved in adult cerebrovascular repair, was highly expressed in astrocytes at birth and then decreased rapidly. Astrocyte-selective ablation ofHmgb1at birth affected astrocyte morphology and endfoot placement, altered distribution of endfoot proteins connexin43 and aquaporin-4, induced transcriptional changes in astrocytes related to cytoskeleton remodeling, and profoundly disrupted endothelial ultrastructure. While lack of astroglialHmgb1did not affect the blood-brain barrier or angiogenesis postnatally, it impaired neurovascular coupling and behavior in adult mice. These findings identify astroglialHmgb1as a key player in postnatal gliovascular maturation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
