A new rabbit model of implant-related biofilm infection: development and evaluation
| Autor: | Hong Zeng, Ding-Xia Shen, Cheng-Bing Chu, Ji-Fang Wang, Hui Wang, Fuzhai Cui |
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| Rok vydání: | 2015 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine 030222 orthopedics Materials science biology medicine.medical_treatment 030106 microbiology Biofilm Total knee arthroplasty biology.organism_classification Condyle 03 medical and health sciences 0302 clinical medicine Staphylococcus epidermidis medicine Rabbit model General Materials Science Implant Saline Biomedical engineering Red fluorescence |
| Zdroj: | Frontiers of Materials Science. 10:80-89 |
| ISSN: | 2095-0268 2095-025X |
| DOI: | 10.1007/s11706-016-0324-1 |
| Popis: | This study is to establish a rabbit model for human prosthetic joint infection and biofilm formation. Thirty-two healthy adult rabbits were randomly divided into four groups and implanted with stainless steel screws and ultra-high molecular weight polyethylene (UHMWPE) washers in the non-articular surface of the femoral lateral condyle of the right hind knees. The rabbit knee joints were inoculated with 1 mL saline containing 0, 102, 103, 104 CFU of Staphylococcus epidermidis (S. epidermidis) isolated from the patient with total knee arthroplasty (TKA) infection, respectively. On the 14th postoperative day, the UHMWPE washers from the optimal 103 CFU group were further examined. The SEM examination showed a typical biofilm construction that circular S. epidermidis were embedded in a mucous-like matrix. In addition, the LCSM examination showed that the biofilm consisted of the polysaccharide stained bright green fluorescence and S. epidermidis radiating red fluorescence. Thus, we successfully create a rabbit model for prosthetic joint infection and biofilm formation, which should be valuable for biofilm studies. |
| Databáze: | OpenAIRE |
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