Expression and localization of the inducible isoform of nitric oxide synthase in nasal polyp epithelium
| Autor: | D.J. Peroni, Philip J. Thompson, Kerrie A Basclain, M.J. Garlepp, D.N. Watkins, P.H. Fisher, R.H. Lewis |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Nasal cavity
Pathology medicine.medical_specialty medicine.medical_treatment Immunology Turbinectomy In situ hybridization respiratory system Biology medicine.disease Nitric oxide Nitric oxide synthase chemistry.chemical_compound medicine.anatomical_structure chemistry otorhinolaryngologic diseases medicine biology.protein Immunology and Allergy Respiratory epithelium Nasal polyps Nasal Turbinate |
| Zdroj: | Clinical & Experimental Allergy. 28:211-219 |
| ISSN: | 0954-7894 |
| DOI: | 10.1046/j.1365-2222.1998.00215.x |
| Popis: | Background The pathogenesis of nasal polyp disease is poorly understood. Recent evidence has suggested that nitric oxide (NO), an endogenous soluble gas vasodilator and inflammatory mediator, may be synthesised within the nasal cavity. Three nitric oxide synthase isoforms have been identified in humans, with the inducible isoform (iNOS) generally expressed in the setting of inflammation. Objective The aim of this study was to detect and localize iNOS expression in nasal polyp tissue, and compare these findings with normal nasal turbinate tissue. Methods We examined the expression and localisation of inducible nitric oxide synthase (iNOS) in human nasal airway specimens from patients undergoing elective nasal turbinectomy (n = 5) or nasal polypectomy (n = 5). iNOS mRNA expression was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) followed by Southern blot analysis and localised by in situ hybridization. Densitometric data were analysed using Student's unpaired t-test. Adjacent sections were also examined for iNOS protein expression by immunohistochemistry. Results Semi-quantitative RT-PCR/Southern analysis of RNA obtained from the 10 surgical specimens demonstrated that iNOS mRNA expression was significantly increased in the five nasal polyps (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|