Monocyte gene expression is effected by route of feeding in healthy human volunteers

Autor:	Daniel J. Bowers, John L. Nosher, Stephen F. Lowry, Marie A. Macor, Michael T. Reddell, Jacqueline E. Calvano, Susette M. Coyle, Hao Liu, Ashwini Kumar, Steven E. Calvano
Rok vydání:	2005
Předmět:	medicine.diagnostic_test business.industry Docking protein 5 Tissue inhibitor of metalloproteinase Molecular biology Fold change Western blot Gene expression Immunology Medicine Surgery DNA microarray business Gene Bradykinin receptor B2
Zdroj:	Journal of the American College of Surgeons. 201:S42
ISSN:	1072-7515
DOI:	10.1016/j.jamcollsurg.2005.06.085
Popis:	RESULTS: Successful overexpression of CuZnSOD was confirmed by western blot and polymerase chain reaction analysis. This validated the model system. The outcome of the Affymetrix microarray analysis was that at a cut-off value of 2 fold change, we identified 27 genes up-regulated and 47 genes down-regulated in response to over-expression of CuZnSOD. Among the novel genes upregulated were the anti-atherogenic genes TIMP3: tissue inhibitor of metalloproteinase 3 and DOK5; docking protein 5. Among the novel genes downregulated were the pro-atherogenic genes HDGF: hepatoma-derived growth factor and BDKRB2 : bradykinin receptor B2.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::404f5c598df8ac1c3bec09a85e08e979 https://doi.org/10.1016/j.jamcollsurg.2005.06.085 Zobrazit plný text záznamu