Near infra-red diode laser for photodynamic tumour therapy using bacteriochlorina

Autor: Carel J. van der Poel, Tom M.A.R. Dubbelman, Jaap A. Van Best, Hans J. Schuitmaker, Jan Fakkel
Rok vydání: 1993
Předmět:
Zdroj: Lasers in Medical Science. 8:157-162
ISSN: 1435-604X
0268-8921
DOI: 10.1007/bf02547871
Popis: Clonogenic cell survivals were performed in order to assess the feasibility of tumour cell kill with an experimental diode laser emitting 250 mW of light at λ = 779 nm using the photosensitizer bacteriochlorina(BCA). The AlGaAs diode laser is based on organometallic vapour epitaxial crystal growth technology. The electrical to optical conversion efficiency amounts to 21% and the beam divergence is 47° by 7.0° full width at half maximum. BCA was proved to be an effective non-toxic photosensitizer in vitro and in vivo. It has a major absorption peak at 760 nm where tissue penetration of light is optimal. Clonogenic T24 human bladder carcinoma cell survivals were photosensitizer concentration and light dose dependent. A 0.1% survival rate was obtained with an illumination intensity of 50 mWcm−2 for 90 s (4.5 Jcm−2) and a BCA concentration of 6 μgml−1. Illumination without BCA at energy levels exceeding the PDT levels with a factor 10, or BCA alone without illumination had no effect on the cells in the clonogenic cell survivals. The combination of BCA with a near infra-red diode laser is most promising for photodynamic tumour therapy as a result of the reliability, compactness and relatively low price of the illumination device, the high transmittance of near infra-red light in tissue and the tumour killing potential of BCA.
Databáze: OpenAIRE
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