| Popis: |
Research in the geothermal regions of Yellowstone National Park has revealed an abundance of microorganisms that inhabit these extreme environments. The description of these microbial populations has been addressed by a number of approaches. One approach was the cultivation and identification of microorganisms using classical microbiological methods (Brock and Darland, 1971; Brock and Freeze, 1969; Brock et al., 1972). Other approaches use molecular methods that identify microorganisms by sequence analysis of nucleic acids extracted from collected biomass (Stahl et al., 1985), polymerase chain reaction (PCR)-amplified rRNA genes from clones and isolates (Barns et al., 1994; Reysenbach et al., 1994; Saul et al., 1993), and cDNA synthesized from small subunit ribosomal RNA (16S rRNA) extracted from collected biomass (Ward et al., 1990). |
