Metabolic and molecular-genetic changes in the liver during carbon tetrachloride intoxication
| Autor: | Samat S. Baygildin, Yana V. Valova, E. F. Repina, Tatyana G. Kutlina, D. O. Karimov, G. F. Mukhammadiyeva |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Biochemistry Chemistry 030220 oncology & carcinogenesis Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Carbon tetrachloride General Medicine Pollution |
| Zdroj: | Hygiene and sanitation. 99:996-1000 |
| ISSN: | 2412-0650 0016-9900 |
| DOI: | 10.47470/0016-9900-2020-99-9-996-1000 |
| Popis: | Introduction. Toxic hepatitis (TH) is a complex and multifaceted disease, the development of which is mediated by a complex of biochemical and molecular genetic interactions. The current understanding of the pathogenesis of TH and, as a consequence, its treatment is based on standardization of the phenotype of the disease, often without taking into account metabolic disorders within the cells. Material and methods. experimental studies were performed on white outbred male rats weighing 200-220 g. A 50% solution of TCM was used as a toxicant. Biochemical studies were performed on a laboratory medical photometer “Stat Fax 3300” using clinical test kits and control materials manufactured by Vector-Best LLC. Liver tissue for histological examination was subjected to the standard histological procedure and paraffin embedding. Sections 5-7 μm thick were stained with hematoxylin-eosin. Gene expression analysis was performed using real-time PCR amplification on a RotorGene instrument (QIAGEN). Statistical processing of experimental data was performed using the Pearson correlation coefficient and one-way analysis of variance (ANOVA). The results were considered reliable at p |
| Databáze: | OpenAIRE |
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