Determination of Half-Maximal Inhibitory Concentration of an Enzyme Inhibitor
| Autor: | Georgios Premetis, Evangelia G. Chronopoulou, Christina Varotsou, Elisavet Ioannou, Nikolaos E. Labrou, Nikolaos Georgakis |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Pharmaceutical drug Drug biology 010405 organic chemistry Drug discovery medicine.medical_treatment media_common.quotation_subject Pharmacology 01 natural sciences 0104 chemical sciences 010104 statistics & probability Enzyme Drug development chemistry Enzyme inhibitor biology.protein medicine Enzyme kinetics 0101 mathematics IC50 media_common |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071601624 |
| DOI: | 10.1007/978-1-0716-0163-1_3 |
| Popis: | Drug development is the process of bringing a new pharmaceutical drug to the market once a lead compound has been identified through the process of drug discovery. Enzymes are one of the most important groups of drug targets; thus, enzyme inhibition is widely used for the treatment of certain disorders. The assessment of an inhibitor against an enzyme is predominantly based on two different parameters: the half-maximal inhibitory concentration (IC50) and the inhibition constant (Ki). This chapter describes an experimental procedure for the determination of the IC50 value of an enzyme inhibitor. The relationship between IC50 and Ki is also discussed. |
| Databáze: | OpenAIRE |
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