Circulating Plasma Albumin mRNA as a Predictor of Liver Injury in Chronic Hepatitis C and Hepatocellular Carcinoma
| Autor: | Mohammed Akl Rady, Tawfik M Abd El Mottaleb, Soumaya Suliman, Waleed N Hassan, Maha Sabawi, Ghada R El Hendawy, Yasser F Thabet, Mervat Mohii |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Liver injury Messenger RNA Pathology medicine.medical_specialty medicine.diagnostic_test business.industry Immunology Albumin Alpha (ethology) medicine.disease digestive system diseases Liver disease Abdominal ultrasonography Hepatocellular carcinoma medicine Immunology and Allergy business Liver function tests |
| Zdroj: | American Journal of Biomedical Sciences. :258-267 |
| ISSN: | 1937-9080 |
| DOI: | 10.5099/aj110400258 |
| Popis: | Background: Analysis of circulating nucleic acids in plasma, such as cell free RNA offers an avenue for non invasive monitoring of a variety of physiological and pathological conditions. Aims: Because albumin is the most abundant protein in the body and is synthesized by the liver, the current study was designed to assess plasma albumin mRNA (ALB mRNA), as a non invasive diagnostic marker of liver injury in chronic HCV (CHC) and hepatocellular carcinoma (HCC). Patients and Methods: The study included 65 patients, 31 patients had CHC and 34 were of HCC as well as 25 healthy control subjects. Patients were subjected to clinical examination, abdominal ultrasonography, CT for HCC cases and laboratory investigations including liver function tests, AFP and plasma albumin mRNA by Real Time- PCR. Results: Patients with CHC and HCC have a significant increase in their plasma ALB mRNA than controls; the higher level was in HCC cases. At a cut-off >935 copies/ml, plasma ALB mRNA can discriminate liver diseased from healthy subjects, with a sensitivity of 81.5%, and specificity of 96%, while, elevated serum levels of ALT had a sensitivity of 32.3%, and specificity of 92%. However, at a cut off >20 ng/ml alpha feto protein (AFP) had a sensitivity of 55.9% and sensitivity of 91.2%.in diagnosis of HCC. Conclusion: ALB mRNA in plasma is liver specific; it is increased in liver disease suggesting liver pathology and may be more diagnostically sensitive than alpha-fetoprotein and Alanine aminotransaminase (ALT) serum levels. Thus, future studies should assess if the plasma concentration of ALB mRNA may be used as therapy monitoring. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|