Determination of berberine and palmatine in Phellodendri Cortex using ion-pair supercritical fluid chromatography on-line coupled with ion-pair supercritical fluid extraction by on-column trapping
| Autor: | Hideki Iwasaki, Kazuhiko Sagara, Shinichi Kakinuma, Hideji Itokawa, Keiichi Suto, Yuji Ito |
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| Rok vydání: | 1997 |
| Předmět: |
Supercritical carbon dioxide
Chromatography Chemistry Organic Chemistry Extraction (chemistry) Supercritical fluid extraction Palmatine General Medicine Biochemistry Analytical Chemistry chemistry.chemical_compound Berberine Supercritical fluid chromatography Methanol Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography A. 786:371-376 |
| ISSN: | 0021-9673 |
| Popis: | An assay of berberine and palmatine in Phellodendri Cortex was established using ion-pair supercritical fluid chromatography (IP-SFC) on-line coupled with ion-pair supercritical fluid extraction (IP-SFE). The on-column method was employed to successfully perform on-line SFE-SFC. With a silica-gel column as a trapping and separation column, berberine and palmatine were extracted and focused at the column head, even if 10% methanol containing dioctyl sodium sulfosuccinate (DSS) was added to the supercritical carbon dioxide as an entrainer. After consecutive extraction and adsorption, berberine and palmatine were desorped and SFC was performed increasing the concentration of DSS methanol solution to 15%. The addition of DSS improved the efficiency of extraction of alkaloids, and the recovery of the analytes of interest was almost the same as that obtained with solvent extraction. Determination of the analytes in Phellodendri Cortex was achieved with only a few mg of crude sample. SFE was completed within ca. 10 min, and SFC was also achieved within ca. 10 min. The consecutive procedures of extraction, concentration and analysis were thus completed within ca. 20 min. Commercial Phellodendri Cortex was tested for determination of the analytes, and results confirmed that our on-line SFE-SFC method can be used for rapid convenient assay of berberine and palmatine in Phellodendri Cortex. |
| Databáze: | OpenAIRE |
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