The Tor and Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32
| Autor: | Takeshi Uchiumi, Tadashi Yamamoto, Yutaka Yoshida, Yusuke Kurihara, Tetsu Saigusa, Yasuyoshi Sakai, Masamune Aihara, Yuichi Matsushima, Xiulian Jin, Masahide Oku, Yoshimasa Aoki, Tomotake Kanki, Dongchon Kang, Yuko Hirota |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Journal of Cell Science. |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.153254 |
| Popis: | When mitophagy is induced in Saccharomyces cerevisiae, the mitochondrial outer membrane protein ScAtg32 interacts with the cytosolic adaptor protein ScAtg11. ScAtg11 then delivers the mitochondria to the pre-autophagosomal structure for autophagic degradation. Despite the importance of ScAtg32 for mitophagy, the expression and functional regulation of ScAtg32 are poorly understood. In this study, we identified and characterized the ScAtg32 homolog in Pichia pastoris (PpAtg32). Interestingly, we found that PpAtg32 was barely expressed before induction of mitophagy and was rapidly expressed after induction of mitophagy by starvation. Additionally, PpAtg32 was phosphorylated when mitophagy was induced. We found that PpAtg32 expression was suppressed by Tor and the downstream PpSin3-PpRpd3 complex. Inhibition of Tor by rapamycin induced PpAtg32 expression, but could neither phosphorylate PpAtg32 nor induce mitophagy. Based on these findings, we conclude that the Tor and PpSin3-PpRpd3-related pathway regulates PpAtg32 expression, but not PpAtg32 phosphorylation. |
| Databáze: | OpenAIRE |
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