Optical dephasing in red fluorescent protein

Autor:	Silvia Völker, S. Bonsma, R. Purchase, J. Gallus, F. Konz
Rok vydání:	2004
Předmět:	Spectral power distribution Chemistry Phonon Dephasing Biophysics General Chemistry Condensed Matter Physics Biochemistry Fluence Power law Atomic and Molecular Physics and Optics Excited state Spectral hole burning Atomic physics Excitation
Zdroj:	Journal of Luminescence. 108:153-157
ISSN:	0022-2313
Popis:	The excited state dynamics of the red fluorescent protein DsRed have been studied by spectral hole-burning at low temperature. The homogeneous line width Γhom of the S1←S0 0–0 transition of the red (R-) form was obtained from MHz-resolution hole widths that were extrapolated to a burning fluence of zero at each temperature. The temperature dependence of Γhom follows a T1.3 power law between 1.2 and 4 K. For T→0, Γhom extrapolates to a value determined by the fluorescence lifetime τfl∼3.5 ns. The spectral distribution of hole depths in the red wing of the excitation spectrum is interpreted in terms of ‘trap’ states for ‘downhill’ energy-transfer within the DsRed tetramer. Furthermore, from simulations of experimentally determined zero-phonon holes and phonon-side holes we obtain for the R-form an electron–phonon coupling strength S of ∼1.25 and a mean phonon frequency νph of ∼50 cm−1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3fda22954b401cd1a0e89b78b86420d6 https://doi.org/10.1016/j.jlumin.2004.01.033 Zobrazit plný text záznamu Full Text from ScienceDirect