Low-Pressure Plasma Application for the Inactivation of the Seed-borne Pathogen Xanthomonas campestris
| Autor: | Kiyotsugu Okada, Tatsuya Misawa, Yuichiro Takai, Hideo Tanimoto, Mitsuo Kawaradani, Tomoko Mishima, Terumi Nishioka, Shin-ichi Kusakari |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology DNA damage Inoculation 030106 microbiology Public Health Environmental and Occupational Health food and beverages Contamination biology.organism_classification Applied Microbiology and Biotechnology Xanthomonas campestris Microbiology 03 medical and health sciences chemistry.chemical_compound Real-time polymerase chain reaction chemistry Seed treatment Pathogen Bacteria |
| Zdroj: | Biocontrol Science. 21:37-43 |
| ISSN: | 1884-0205 1342-4815 |
| Popis: | The aim of this study was to investigate the effect of low-pressure plasma treatment on seed disinfection and the possible mechanisms underlying this effect. Seed-borne disease refers to plant diseases that are transmitted by seeds; seed disinfection is an important technique for prevention of such diseases. In this study, the effectiveness of low-pressure plasma treatment in the inactivation of the seed-borne plant pathogenic bacterium, Xanthomonas campestris, inoculated on cruciferous seeds, was evaluated. The highest inactivation effect was observed when the treatment voltage and argon gas flow rate were 5.5 kV and 0.5 L/min, respectively. The viable cell number of X. campestris was 6.6 log cfu/seed before plasma treatment, and decreased by 3.9 log after 5 min of treatment and by 6.6 log after 40 min. Ethidium monoazide treatment and quantitative real-time PCR results indicated that both the cell membrane and target DNA region were damaged following 5 min of plasma treatment. Although both heat and ozone were generated during the plasma treatment, the contribution of both factors to the inactivation of X. campestris was small by itself in our low-pressure plasma system. Overall, we have shown that our low-pressure plasma system has great applicability to controlling plant pathogenic bacterium contamination of seeds. |
| Databáze: | OpenAIRE |
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