Host Alternative NADH:Ubiquinone Oxidoreductase Serves as a Susceptibility Factor to Promote Pathogenesis of Rhizoctonia solani in Plants
| Autor: | Gopaljee Jha, Ravi Kant, Srayan Ghosh, Kriti Tyagi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Reactive oxygen species biology Host (biology) food and beverages Plant Science biology.organism_classification 01 natural sciences Enzyme assay Microbiology Rhizoctonia solani Pathogenesis 03 medical and health sciences 030104 developmental biology chemistry Oxidoreductase biology.protein Gene silencing Agronomy and Crop Science Gene 010606 plant biology & botany |
| Zdroj: | Phytopathology®. 109:1741-1750 |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-02-19-0055-r |
| Popis: | Phytopathogens have evolved mechanisms to utilize host genes (commonly known as susceptibility factors) to promote their pathogenesis. Rhizoctonia solani is a highly destructive fungal pathogen of various plants, including rice. We previously reported rice genes that were differentially regulated during R. solani pathogenesis. In this study, we analyzed the role of tomato homologs of two rice genes, isoflavone reductase (IFR) and alternative NADH:ubiquinone oxidoreductase (NUOR), as potential susceptibility factors for R. solani. Virus-induced gene silencing of NUOR in tomato resulted in compromised susceptibility against R. solani, whereas IFR-silenced plants demonstrated susceptibility similar to that of control plants. NUOR silencing in tomato led to homogenous accumulation of reactive oxygen species (optimum range) upon R. solani infection. In addition, the expression and enzyme activity of some host defense and antioxidant genes was enhanced, whereas H2O2 content, lipid peroxidation, and electrolyte leakage were reduced in NUOR-silenced plants. Similarly, transient silencing of OsNUOR provided tolerance against R. solani infection in rice. Overall, the data presented in this study suggest that NUOR serves as a host susceptibility factor to promote R. solani pathogenesis. |
| Databáze: | OpenAIRE |
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