| Popis: |
The purpose of this research is to recognise antiquorum sensing and antibiofilm activity against biofilm-forming bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes, Enterobacter aerogenes, and Bacillus cereus) and virulence factors in Pseudomonas extracts isolated from petroleum-oil contaminated side. The extracts were tested for anti-QS activity on Chromobacterium violaceum ATCC 12472 in double-fold dilutions of 50, 25, 12.5 and 6.25 mg/mL. The inhibition of violecein production from extracts at 50, 25, 12.5 and 6.25 mg/mL dilutions were the best determined between 30.2% and 72.5% at the dilution of 50 mg/mL. The crude extracts from the P9 (78%), P12 (88%) and P13 (75%) isolates, B. cereus 709 Roma have the best biofilm inhibition activity. At the same time, the crude extracts of P3, P8 and P13 isolate, E. aerogenes (91%), (85%) and (70%) and showed the highest biofilm inhibition activity, respectively. Multidrug-resistant bacteria commonly use cell-to-cell communication leading to biofilm formation as one of the mechanisms of resistance development. Quorum sensing inhibition (QSI) is an effective approach to prevent biofilm formation. A gram-negative bacterium, 11 P. aeruginosa, was isolated from soil contaminated with petroleum oil. Bacterial crude extracts showed QSI activity in the biosensor plate containing the reference strain C. violaceum ATCC 12742. A reduction of approximately 98% in violacein production was detected with the reference strain C. violaceum ATCC 12742. The aim of this research is to screen anti-quorum sensing activity and inhibited biofilm activity of Pseudomonas aeruginosa isolated from soil contaminated with petroleum oil and to measure the antibiofilm activities by biofilm forming bacteria (S. aureus, E. coli, P. aeruginosa, E. aerogenes, L. monocytogenes and B. cereus 709 Roma). |
