| Autor: |
John M. Tarbell, Hanjoong Jo, Theodore M. Hollis, Randal O. Dull |
| Rok vydání: |
1990 |
| Předmět: |
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| Zdroj: |
Biofluid Mechanics ISBN: 9783540527305 |
| DOI: |
10.1007/978-3-642-52338-0_22 |
| Popis: |
Endothelial cells regulate transmural volume flux and permeability of the vascular wall to macromolecules. Alteration of wall permeability may be an early event in atherogenesis. In order to study transport properties of the endothelial monolayer in isolation from the underlying structures of the arterial wall, we developed an in vitro model employing bovine aortic endothelial cells grown on polycarbonate filters. Hydraulic conductivity (Lp) was measured under static conditions (zero wall shear stress) by a bubble tracking technique in both the presence and absence of albumin in the culture media. Endothelial permeability to flourescently labelled albumin (Pe) was measured under static conditions and during exposure to steady wall shear stress of 1 or 10 dynes/cm2 in a parallel plate flow chamber with 1% albumin in the culture media on both sides of the monolayer. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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