Approaches for yield increase of β-cyclodextrin formed by cyclodextrin glucanotransferase from Bacillus megaterium
| Autor: | Ivan Pishtiyski, Georgi Dobrev, Veselin Stanchev, Boriana Zhekova |
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| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Chromatography Cyclodextrin biology Physiology Chemistry Starch technology industry and agriculture General Medicine biology.organism_classification Applied Microbiology and Biotechnology Toluene carbohydrates (lipids) chemistry.chemical_compound Product inhibition Yield (chemistry) polycyclic compounds lipids (amino acids peptides and proteins) Selectivity Biotechnology Nuclear chemistry Cyclomaltodextrin glucanotransferase Bacillus megaterium |
| Zdroj: | World Journal of Microbiology and Biotechnology. 25:1043-1049 |
| ISSN: | 1573-0972 0959-3993 |
| Popis: | Two methods for increasing β-cyclodextrin yield, achieved with cyclodextrin glucanotransferase from Bacillus megaterium were investigated. A membrane process was performed, allowing a reuse of the enzyme. A process for simultaneous production and isolation of β-cyclodextrin in the presence of complexing agents was conducted. The β-cyclodextrin yield was increased twofold, when the product was precipitated with trichloroethylene or toluene. A change in the product selectivity of cyclodextrin glucanotransferase occurred, resulting in an increase in the relative amount of β-cyclodextrin up to 90% of all cyclodextrins formed. Yield increase was due to the removal of product inhibition and the coupling activity of the enzyme, which limited the full conversion of starch. The isolated cyclodextrin products contained 75–79% β-cyclodextrin, and 4–6% each of α-, and γ-cyclodextrins. |
| Databáze: | OpenAIRE |
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