Nucleotide Pools and Physiological Consequences of Heat Shock in Euglena Cells
| Autor: | Régis Calvayrac, Jean-Loup Bomsel, Mahmoud Edjlali |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
High energy Euglena gracilis biology ved/biology ved/biology.organism_classification_rank.species General Medicine biology.organism_classification Euglena Biochemistry chemistry Shock (circulatory) Respiration Etiolation medicine Biophysics Nucleotide Energy charge medicine.symptom |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Physiology. 117:171-176 |
| ISSN: | 0300-9629 |
| Popis: | Etiolated cells of Euglena gracilis, grown at 26°C in the dark, were taken at different growth phases and submitted to heat shocks (up to 2 hr) at temperatures ranging from 33 to 42°C. Their volumes and mortality increased rapidly with temperature and the duration of the shock. On the contrary, the apparent gas exchange during respiration slowed suddenly at temperatures higher than 38°C. A direct and positive correlation appears to exist between high Energy Charges, catalatic activities, and the persistence of respiration. After only 1 hr at 40 or 42°C, respiration ceased, while cell catalatic activities and Energy Charge collapsed. The adenylic pool of treated cells increased independently of the growth phase of cells. This evolution was parallel to that of cell volume. These results are discussed with respect to variations in catalatic activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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