Continuous-flow enzyme immunoassay for thyroxine in serum

Autor:	N J Tarsa, G DiBenedetto, J P Nolan
Rok vydání:	1981
Předmět:	chemistry.chemical_classification Chromatography medicine.diagnostic_test biology Bilirubin Biochemistry (medical) Clinical Biochemistry Radioimmunoassay AutoAnalyzer Enzyme assay chemistry.chemical_compound Enzyme chemistry Immunoassay medicine biology.protein Euthyroid Hemoglobin
Zdroj:	Clinical Chemistry. 27:738-741
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/27.5.738
Popis:	We have evaluated a fully automated thyroxine assay involving the use of a homogeneous enzyme assay and a Technicon AutoAnalyzer II continuous flow system. Comparison by regression analysis with a thyroxine radioimmunoassay kit method gave a slope of 0.82 and a y-intercept of 7.81 micrograms/L (SE 0.86 microgram/L, r = 0.95). Within-run precision yielded CVs of 1.0-6.1%, between-day CVs were 2.0-14.4%; within-day precision showed a mean variance of 7.81 micrograms/L. Mean analytical recovery was 96.1%. Bilirubin, hemoglobin, and lipemia interfered with quantitation of results when their concentrations exceeded 50 mg/L, 300 mg/L, and 5 g/L, respectively. The reference interval for euthyroid status was calculated to be 50-110 micrograms/L. Sensitivity was 5.0 micrograms/L with a mean carryover of 1.65%. Current reagent and labor costs for enzyme immunoassay ($0.40) were less than for the manual radioimmunoassay procedure ($0.40) were less than for the manual radioimmunoassay procedure ($1.70). The assay is economical, simple to perform, and amenable to high-throughput thyroid screening in the routine laboratory.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3f6942fb95779c97c3e98d48f0a4b9c9 https://doi.org/10.1093/clinchem/27.5.738 Zobrazit plný text záznamu