Quantitative Determination of Carthamin in Carthamus Red by 1H-NMR Spectroscopy

Autor:	Fan Bai, Naoki Sugimoto, Takamitsu Yoshida, Takeshi Yamazaki, Hajime Mizukami, Kazuyoshi Terasaka, Hiroshi Akiyama, Setsuko Kato
Rok vydání:	2013
Předmět:	Detection limit Chromatography biology Calibration curve Carthamus General Chemistry General Medicine biology.organism_classification High-performance liquid chromatography chemistry.chemical_compound Certified reference materials chemistry Drug Discovery Botany Spectroscopy Colorimetry Carthamin
Zdroj:	Chemical and Pharmaceutical Bulletin. 61:1264-1268
ISSN:	1347-5223 0009-2363
DOI:	10.1248/cpb.c13-00533
Popis:	Carthamus Red is a food colorant prepared from the petals of Carthamus tinctorius (Asteraceae) whose major pigment is carthamin. Since an authentic carthamin standard is difficult to obtain commercially for the preparation of calibration curves in HPLC assays, we applied (1)H-NMR spectroscopy to the quantitative determination of carthamin in commercial preparations of Carthamus Red. Carthamus Red was repeatedly extracted in methanol and the extract was dissolved in pyridine-d(5) containing hexamethyldisilane (HMD) prior to (1)H-NMR spectroscopic analysis. The carthamin contents were calculated from the ratios of singlet signal intensities at approximately σ: 9.3 derived from H-16 of carthamin to those of the HMD signal at σ: 0. The integral ratios exhibited good repeatability among NMR spectroscopic analyses. Both the intra-day and inter-day assay variations had coefficients of variation of
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3f5d755ad41459944bbaab1b35c81419 https://doi.org/10.1248/cpb.c13-00533 Zobrazit plný text záznamu