α-Mannosidase from rat epididymal fluid is a ligand for phosphomannosyl receptors on the sperm surface

Autor:	L. S. Gutierrez, Francisco Bertini, Silvia A. Belmonte, Miguel A. Sosa, Challa A
Rok vydání:	2002
Předmět:	chemistry.chemical_classification Mannosidase Extracellular transport Urology Endocrinology Diabetes and Metabolism Fructose Biology Epididymis Sperm chemistry.chemical_compound Enzyme medicine.anatomical_structure Reproductive Medicine Biochemistry chemistry medicine Phosphorylation Receptor
Zdroj:	International Journal of Andrology. 21:277-282
ISSN:	0105-6263
DOI:	10.1046/j.1365-2605.1998.00124.x
Popis:	This study demonstrates that alpha-mannosidase from rat epididymal fluid is a ligand for phosphomannosyl receptors on the sperm surface. This enzyme was bound to intact epididymal spermatozoa with high affinity and in saturable form, and the binding was inhibited by mannose-6-phosphate but not by phosphorylated derivatives of fructose. Treatment of the enzyme with sodium periodate inhibited the binding of alpha-mannosidase, confirming that a carbohydrate residue is involved in the interaction with spermatozoa. Evidence is also presented that the cation-independent phosphomannosyl receptors are responsible for the interaction with alpha-mannosidase. These findings suggest a new role for extracellular transport mediated by the mannose-6-phosphate receptor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3f55e9096d83b5a0216ba298fd07af80 https://doi.org/10.1046/j.1365-2605.1998.00124.x Zobrazit plný text záznamu Plný text