A Carboxy-terminal Smarcb1 Point Mutation Induces Hydrocephalus Formation and Affects AP-1 and Neuronal Signalling Pathways in Mice

Autor: Aliska K. Brugmans, Carolin Walter, Natalia Moreno, Carolin Göbel, Dörthe Holdhof, Flavia W. de Faria, Marc Hotfilder, Daniela Jeising, Michael C. Frühwald, Boris V. Skryabin, Timofey S. Rozhdestvensky, Lydia Wachsmuth, Cornelius Faber, Martin Dugas, Julian Varghese, Ulrich Schüller, Thomas K. Albert, Kornelius Kerl
Rok vydání: 2023
Předmět:
Zdroj: Cellular and Molecular Neurobiology.
ISSN: 1573-6830
0272-4340
DOI: 10.1007/s10571-023-01361-5
Popis: The BAF (BRG1/BRM-associated factor) chromatin remodelling complex is essential for the regulation of DNA accessibility and gene expression during neuronal differentiation. Mutations of its core subunit SMARCB1 result in a broad spectrum of pathologies, including aggressive rhabdoid tumours or neurodevelopmental disorders. Other mouse models have addressed the influence of a homo- or heterozygous loss of Smarcb1, yet the impact of specific non-truncating mutations remains poorly understood. Here, we have established a new mouse model for the carboxy-terminal Smarcb1 c.1148del point mutation, which leads to the synthesis of elongated SMARCB1 proteins. We have investigated its impact on brain development in mice using magnetic resonance imaging, histology, and single-cell RNA sequencing. During adolescence, Smarcb11148del/1148del mice demonstrated rather slow weight gain and frequently developed hydrocephalus including enlarged lateral ventricles. In embryonic and neonatal stages, mutant brains did not differ anatomically and histologically from wild-type controls. Single-cell RNA sequencing of brains from newborn mutant mice revealed that a complete brain including all cell types of a physiologic mouse brain is formed despite the SMARCB1 mutation. However, neuronal signalling appeared disturbed in newborn mice, since genes of the AP-1 transcription factor family and neurite outgrowth-related transcripts were downregulated. These findings support the important role of SMARCB1 in neurodevelopment and extend the knowledge of different Smarcb1 mutations and their associated phenotypes.
Databáze: OpenAIRE
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