Cryopreservation of Sperm as a Means to Store Salmonid Germ Plasm and to Transfer Genes from Wild Fish to Hatchery Populations

Autor:	M. J. Levanduski, J. G. Cloud, W. H. Miller
Rok vydání:	1990
Předmět:	endocrine system Fish migration animal structures urogenital system Zoology Semen Aquatic animal Biology Sperm Cryopreservation Hatchery Fishery Fish hatchery Rainbow trout General Agricultural and Biological Sciences
Zdroj:	The Progressive Fish-Culturist. 52:51-53
ISSN:	1548-8640 0033-0779
DOI:	10.1577/1548-8640(1990)052<0051:cosaam>2.3.co;2
Popis:	The objective of this investigation was to determine if cryopreservation of salmonid sperm can be used successfully under normal field conditions. Sperm were collected from natural or wild steelhead (anadromous rainbow trout [Oncorhynchus mykiss]) trapped on two Lochsa River, Idaho, tributaries during spring 1987. Semen was frozen in liquid nitrogen and stored until spring 1988 (approximately 11 months), when it was thawed, and the sperm were used to fertilize eggs ofstock females from Dworshak National Fish Hatchery, Ahsahka, Idaho. Of the 44,676 eggs used from six females, 10,404 developed to the eyed stage and 10,254 embryos hatched.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3f33eb05d165822897137d56bb15cf75 https://doi.org/10.1577/1548-8640(1990)052<0051:cosaam>2.3.co Zobrazit plný text záznamu Plný text