Sequestration of Stigmasterol and β-Sitosterol from Ethanolic Extract of Kam Sabut (Croton caudatus Geiseler) Leaves

Autor:	Raghuvir H Gaonkar, H. Lalhenmawia, Sharubam Victoria Devi, Longjam Shantabi, Ganesh Chandra Jagetia
Rok vydání:	2021
Předmět:	chemistry.chemical_compound Stigmasterol Traditional medicine Chemistry General Chemistry Croton caudatus
Zdroj:	Asian Journal of Chemistry. 33:2851-2856
ISSN:	0975-427X 0970-7077
Popis:	Croton caudatus (Kam sabut) has been traditionally used to treat cancer in northeastern India and earlier studies reported the anticancer activity of ethanolic extract of C. caudatus leaves in HeLa cells in vitro and Swiss albino mice transplanted with Dalton’s ascites lymphoma. Therefore, isolation of stigmasterol and β-sitosterol from the ethanolic extract of C. caudatus Geiseler leaves was carried out. Stigmasterol and β-sitosterol were isolated from the ethanolic extract of dried leaves of C. caudatus by column chromatography. The fractions were purified further on Sephadex® LH-20 column chromatography and reverse-phase HPLC. The isolated phytosterols were characterized and quantified by IR, 1H & 13 CNMR, mass spectroscopy and HPLC. This has led to the separation of 100 mg stigmasterol and 80 mg β-sitosterol. Stigmasterol and β-sitosterol have been isolated from the ethanolic extract of C. caudatus leaves for the first time.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3f3314ec171a179d250c0e810dd9057f https://doi.org/10.14233/ajchem.2021.23426 Zobrazit plný text záznamu