Studies on the Cytotoxic Action of Silica Dusts on Macrophages in Vitro
| Autor: | Enrico C. Vigliani, Gian Carlo Secchi, B. Pernis, Elena Parazzi |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
chemistry.chemical_classification
biology Silicon dioxide Aldolase A Public Health Environmental and Occupational Health Acid phosphatase Fructose-bisphosphate aldolase In vitro chemistry.chemical_compound Enzyme chemistry Biochemistry Lactate dehydrogenase biology.protein Environmental Chemistry Cytotoxicity General Environmental Science |
| Zdroj: | Archives of Environmental Health: An International Journal. 17:850-859 |
| ISSN: | 0003-9896 |
| DOI: | 10.1080/00039896.1968.10665341 |
| Popis: | Experiments were performed in order to investigate the site of cellular damage induced by silica psrticles in vitro to peritoneal guinea pig macrophages. Considerable amounts of cytoplasmic enzymes, lactate dehydrogenase and aldolase, were released into the medium a short time after addition of silica to the cultures, whereas lysosomal enzymes, acid phosphatase and RNAase, were detected only after more prolonged incubation and in lower quantities. Correspondingly, loss of fluorochromasia from macrophages occurred in a matter of minutes after contact with silica particles. When coarse, nonphagocytabte silica particles were used, a rapid loss of fluorochromasia and early release of cytoplasmic enzymes were also seen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|