Fabrication of patterned micromuscles with high activity for powering biohybrid microdevices
| Autor: | Jungyul Park, Seok Chang Ryu, Kahp Y. Suh, Seung Kyu Choi, Sangho Lee, Deok Ho Kim, Byungkyu Kim, Sukho Park, Pilnam Kim |
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| Rok vydání: | 2006 |
| Předmět: |
Materials science
Metals and Alloys Cardiac muscle Motility Nanotechnology Polyethylene glycol Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound medicine.anatomical_structure chemistry PEG ratio Materials Chemistry medicine Myocyte Electrical and Electronic Engineering Cell adhesion Instrumentation Micropatterning Biomedical engineering Microfabrication |
| Zdroj: | Sensors and Actuators B: Chemical. 117:391-400 |
| ISSN: | 0925-4005 |
| Popis: | In this paper, we report a microfabrication approach to patterned three-dimensional growth of muscle cells for powering biohybrid microdevices. The microwells confined by adhesion-resistant polyethylene glycol (PEG) microstructures were patterned on SiO2-based substrate with controllable surface topography for muscle cell culturing. The morphology and motility of neonatal rat cardiac myocytes patterned within microwells were analyzed with different topographical heights of the PEG barrier. It was found that isolated aggregates of cardiac muscles showed various beating activities depending on the morphology and the number of cells. Furthermore, three-dimensionally grown cardiac muscle cells mediated by a higher physical barrier of PEG microstructures generated higher contraction force with faster beating frequency than those of cells attached to the collagen-coated surface on the culture dish (control), suggesting that control over surface topography of microwells for cell growth would be potentially useful in engineering cell motors. Thus the technique presented here would provide a valuable platform for optimizing the activity and functions of patterned muscle cells in building up integrated bioactuated microdevices. |
| Databáze: | OpenAIRE |
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