The application of high-performance liquid chromatography humic acid columns in determination ofKocof polycyclic aromatic compounds

Autor:	Kristoffer E. N. Jonassen, Torben Nielsen, Poul Erik Hansen
Rok vydání:	2003
Předmět:	chemistry.chemical_classification Partition coefficient Total organic carbon Hydrocarbon Chromatography Chemistry Health Toxicology and Mutagenesis Environmental Chemistry Humic acid Sorption Electrolyte High-performance liquid chromatography Soil contamination
Zdroj:	Environmental Toxicology and Chemistry. 22:741-745
ISSN:	1552-8618 0730-7268
Popis:	An improvement of a method for determination of the distribution coefficient for polycyclic aromatic compounds (PAC) between organic carbon and water (Koc) by high-performance liquid chromatography (HPLC) is presented in this paper. By use of silica-based HPLC columns with chemically immobilized humic acid (Aldrich, Zigma-Aldrich, Taufkirchen, Germany), the retention of PAC to humic acid can be measured, and the results can, when they are compared to those from a blank column, be used to a direct determination of Koc. These values agree quite well with literature Koc values for two- to four-ringed PAC. Our work has shown that it is possible to use this method to investigate how environmental parameters like electrolyte concentration, pH, and temperature affect the sorption of PAC to humic acid. Thermodynamic parameters can be estimated from variable temperature experiments. The advantage of the method is its cost and manpower effectiveness.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3f1c1f991e14e96b8560e76c6819af3f https://doi.org/10.1002/etc.5620220410 Zobrazit plný text záznamu Plný text